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BCR Practices and Procedures
Frozen Microbiology Samples
- Frozen MBIO samples are usually shipped directly from the ship to the investigators or, in the case of Routine Microbiology Samples, to the repository. The samples are packed in coolers on dry ice. As soon as they are received, the samples are inventoried and transferred to a –80°C chest freezer. After the moratorium, information detailing the sample position is published online.
- In case of a power outage or mechanical failure, the freezers have audible temperature alarms that sound when the temperature rises above –70°C. The freezers are also attached to a “sensa-phone system” that notifies curatorial staff, by mobile phone, of a high-temperature situation or power failure. An empty freezer is maintained at –80°C temperature in case samples need to be quickly transferred from a defective freezer.
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Whole-Round Samples
Whole-round samples are collected for special analysis (e.g., interstitial water analysis, microbiology sampling) and pre-defined purposes (e.g., "community" whole rounds proposed early by science party members). Intervals of whole rounds depend on the pre-defined purposes of sampling and type of special analyses applied. The Sample Allocation Committee (SAC) is responsible for developing a strategy for whole-round sampling in the early stages of expedition planning and for including a clear description of the whole-round sampling strategy in the IODP Scientific Prospectus.
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